Phospho-erk5
WebPC12 cells transfected with pcDNA3-HA-PTP-SL WT, CS, or mock DNA were serum-starved for 24 h followed by stimulation with 30 ng/ml EGF for 5 min. Endogenous ERK5 was … WebWestern blot - Anti-ERK5 (phospho T218 + Y220) antibody (ab5686) Peptide Competition: Extracts prepared from HEK293 cells transiently transfected with plasmids expressing …
Phospho-erk5
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WebFeb 4, 2016 · The pre-B-cell receptor (pre-BCR) is an important checkpoint of B-cell development and is essential for a pre-B cell to traverse into an immature B cell. Here, we show that activation of myocyte enhancer factor 2 (Mef2) transcription factors (TFs) by the pre-BCR is necessary for initiating the subsequent genetic network. WebPhospho-Erk5 (Thr218/Tyr220) Antibody detects immunoprecipitated or transfected levels of Erk5 phosphorylated at threonine 218 and tyrosine 220. This antibody cross-reacts with …
WebOct 11, 2024 · In addition, ERK5 activation was detected by a band shift which is a characteristic of phosphorylated ERK5. However, flow-induced Akt phosphorylation was not affected by PAR-1 knockdown using PAR ... WebPhospho-ERK5 (Thr218/Tyr220) Assay. Increased signal is observed with the titration of ERK5 positive cell lysate. Signal for negative lysate remains low throughout the titration. The p Phospho-ERK5 (Thr218/Tyr220) Assay provides a quantitative measure of the data obtained with the traditional Western blot.
WebERK5 MAP kinase cascade has a crucial functions like- induction of special form of cardiac hypertrophy, enhanced neuronal survival and early steps of muscle cell differentiation. Anti-phospho-ERK5 (pThr 218 /pTyr 220) antibody can be used in immunostaining. Rabbit anti- phospho-ERK5 (pThr 218 /pTyr 220) antibody reacts specifically with ERK5 ... WebPhospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when phosphorylated either individually or dually at Thr202 and Tyr204 of Erk1 …
WebFeb 19, 2009 · The purpose of the present study was to investigate the role of myocyte enhancer binding factor 2C (MEF2C), a common substrate of p38 kinase and extracellular …
WebERK5/MAPK7 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5. css image fixed sizeWebMSD phosphoprotein assays provide a rapid and convenient methodfor measuring the levels of protein targets within a single, small-volume sample. The assays are available in both singleplex and multi plex formats. In a singleplex assay, an antibody for a specific protein target is coated on one electrode (or “spot”) per well. earliest symptoms of parkinson\u0027s diseaseWebERK5 is a member of the mitogen-activated protein kinase (MAPK) family that, after stimulation, is activated selectively by dual phosphorylation in the TEY motif by MAPK … css image fit widthWebERK5 has a large C-terminal-half that contains a transcriptional activation domain. However, it has remained unclear how its transcriptional activation activity is regulated. ... Antibodies—Antibodies were purchased as follows: rabbit anti-phospho-ERK5 antibody from Cell Signaling; mouse anti-Myc (9E10) and mouse anti-HA (F-7) antibodies from ... css image float rightWebDec 17, 2013 · ERK5 phosphorylation was assessed in the immunoprecipitate using a phosphospecific antibody. Data (mean +/- SEM of 3 independent experiments) were normalised using HA-ERK5 as loading control and expressed as fold-induction over basal conditions (*p<0.05, two-tailed T-test). earliest taxes can be filedWebAnti-phospho-ERK5 (pThr218/pTyr220) antibody produced in rabbit (Anti-BMK1); affinity isolated antibody, buffered aqueous glycerol solution; Each vial contains enough material for 10 blots; Extracellular Signal-Regulated Kinases … css image floatWebPhospho-ERK5 (Thr218/Tyr220) Assay. Increased signal is observed with the titration of ERK5 positive cell lysate. Signal for negative lysate remains low throughout the titration. … earliest taxes can be filed 2021